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KMID : 1094720000050040275
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 4 p.275 ~ p.287
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Fermentation strategies for recombinant protein expression in the methylotrophic yeast Pichia pastoris
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Zhang Wen-Hui
Inan Mehmet
Meagher Michael M.
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Abstract
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Fermentation strategies for recombinant protein production inPichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.
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KEYWORD
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Pichia pastoris, methylotrophic yeast, fed-batch growth, fermentation optimization, growth modeling, recombinant protein expression
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